Biotin functional magnetic microparticles (PuriMag G- Biotin Beads)
- BrandPuriMag
- TypePuriMag G Series
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Ordering information |
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Name |
Cat. No. |
Vol. |
Scheme |
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G-Botin |
PMG016-2 |
2 ml |
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1. Overview
PuriMag? G-Biotin are biotin-conjugated magnetic nanoparticles designed for the magnetic isolation of avidin-, neutravidin-, or streptavidin-labeled components. These beads feature a large surface area and deliver high capture efficiency in binding assays.
(Note: Ideal for pull-down assays, diagnostic applications, and target purification workflows requiring biotin-streptavidin affinity systems.)
2. product description
Product Specifications
Description
Polymer coated Fe3O4 nanoparticles
Particle Size
200 nm
Number of Beads
~1.7×1010 beads/mg
Matrix
Proprietary polymer
Functional group
Streptavidin group
Group density
~150 μmol biotin / g Beads
Binding capacity
≥30 μg streptavidin / mg Beads
Magnetization
60~70 EMU/g
Formulation
10mg/mL in 25 mM tris-HCl, pH 7.4
Storage
1 year at 2~8 ℃. Do not freeze.
3. Instructions for Use
A. Materials Provided
Biotin Magnetic Beads, 10 mg/mL
B. Additional Materials (Not Provided)
1.Binding/Wash Buffer: TBS with 0.05% Tween-20
2.Elution Buffer: 8M Guanidine HCl, pH 1.5
C. Isolation Procedure
1.Pipette 50 μL (0.5 mg) beads per tube. Add 1 mL Binding Buffer to wash beads.
2.Perform magnetic separation for 2 min or until supernatant clears.
3.Aspirate and discard supernatant. Add 1 mL Binding Buffer for a second wash.
4.Repeat Step 2. Discard supernatant.
5.Resuspend beads in 450 μL Binding Buffer.
6.Add 50 μL serum or cell culture supernatant to beads.
Note: Sample volume may be modified. If <500 μL, dilute to 500 μL final volume with Binding Buffer.
7.Mix gently via vortex or rotator for 30 min.
8.Separate magnetically for 2 min or until supernatant clears.
9.Remove and discard supernatant.
10.Add 500 μL Binding Buffer to wash away unbound proteins.
11.Repeat Steps 8-9. Discard supernatant.
12.Add 100 μL Elution Buffer to beads and mix thoroughly.
13.Incubate at RT for 10 min with occasional gentle agitation/vortexing.
14.Desalt or dialyze eluted samples into an appropriate buffer.
(For research use only!)
