无码人妻久久一区二区三区免费丨,91精品欧美久久久久久,色肉色伦交av色肉色伦,操逼贱货调教操骚逼视频

Your Position: Home > Product > Chemical Biology

Hydrazide group functional magnetic microparticles (PuriMag G-Hydrazide Beads)

2025/6/17 viewers
  • BrandPuriMag
  • TypePuriMag G Series
  • order
Introduction

Ordering information

Name

Cat. No.

Vol.

Scheme

G-Hydrazide

PMG042-2

PMG042-5

2 ml

5 ml

1. Overview

PuriMag? G-Hydrazide, Hydrazide-Activated Magnetic Nanoparticles are uniform, polymer-coated superparamagnetic nanoparticles. Their hydrophilic surface ensures excellent dispersibilitylow non-specific adsorption, and easy handling in diverse buffers. The beads feature a high-density surface coating of hydrazide functional groups, enabling site-specific covalent immobilization of aldehyde/ketone-containing ligands (e.g., antibodies, lectins, glycoproteins, carbohydrates).

Key Applications:

    Glycoprotein immobilization via periodate-oxidized carbohydrate aldehydes

    Ideal for polyclonal antibody coupling through Fc-region carbohydrates, preserving antigen-binding orientation

    Compatible with carbohydrate-rich monoclonal antibodies

Immobilization Chemistry:

    Periodate oxidation of cis-diol groups in glycans generates aldehydes.

    Spontaneous reaction between aldehydes and bead hydrazide groups forms stable hydrazone bonds.

Features & Advantages:

    Efficient covalent coupling

    Stable hydrazone bonds with minimal ligand leakage

    Generates reusable immunoaffinity matrices

    Low non-specific binding

    Capacity: 1–20 mg protein or 0.1–2 mg peptide per gram beads

    Long hydrophilic spacers minimize steric hindrance

Operational Flexibility:

    Adaptable coupling time/temperature

    Compatible with affinity chromatography workflows

Schematic Diagram of Bead Coupling Mechanism:



2. product description

Product Specifications

Description

Polymer coated Fe3O4 nanoparticles

Particle Size

200 nm

Number of Beads

~1.7×1010 beads/mg

Matrix

Proprietary polymer

Functional group

Hydrazide groups

Group density

~300 μmole / g of Beads

Magnetization

60~70 EMU/g

Formulation

10mg/mL in in 1M NaCl and 0.02% NaN3

Stability

pH 3.5~10, 4~50 ℃, most organic solvents

Storage

1 year at 2~8 ℃. Do not freeze.

3. Instructions for Use

Note: This protocol exemplifies coupling proteins/peptides to PuriMag? G-Hydrazide beads. Titration-based bead optimization for individual applications is strongly recommended. Scale proportionally. Avoid Tris or buffers containing primary amines/sugars, as they compete with coupling.

A. Required Materials

    1.Magnetic Racks:

        For 12×1.5–2 mL tubes (Mrack02)

        For 2×50 mL or 2×15 mL tubes (Mrack03)

    2.Coupling Buffer: 0.1 M Sodium Phosphate, pH 7.0 or 0.1 M Sodium Acetate, pH 5.6

    3.Oxidizing Agent: Sodium meta-Periodate (NaIO?)

    4.Desalting Column: Sephadex G-25

    5.Wash Buffer: 1 M NaCl

B. Protein Coupling
B-1. Glycoprotein Oxidation
Light-sensitive reaction – perform in dark.

    1.Dissolve/dilute 0.5–10 mg glycoprotein in 1 mL coupling buffer.

        (If pre-suspended in other buffers, exchange via dialysis/desalting.)

    2.Add protein solution to amber vial containing 2 mg NaIO? (10 mM final). Gently agitate to dissolve oxidizer.

    3.Incubate with gentle agitation at RT in dark for 30 min.

    4.Terminate reaction. Remove unreacted NaIO? via Sephadex G-25 desalting/buffer exchange:

            Equilibrate column with 5 mL coupling buffer.

            Load oxidized sample onto column bed.

            Wash with 0.5 mL coupling buffer into bed.

            Elute with 2 mL coupling buffer; collect eluate (typically 1.5–2 mL).

B-2. Coupling to Hydrazide-Terminated Beads
*Note: Store beads as 30 mg/mL suspension in 1 mM EDTA at 4°C. Vortex vigorously before use.*

    1.Transfer 0.5 mL fully resuspended beads (30 mg/mL) to microcentrifuge tube.

    2.Place tube in magnetic separator for 1–3 min. Discard supernatant while tube remains in separator.

    3.Remove tube. Resuspend beads in 1.5 mL coupling buffer.

    4.Repeat Steps 2–3 three times (total 4 washes).

    5.Resuspend beads in 750 μL coupling buffer.

    6.Add 250 μL oxidized protein solution. Incubate at RT for ≥6 h.

            *Note: Optimize protein concentration empirically. For 10 mg beads, start with 100–250 μg/mL.*

    7.Wash beads 3× with 1 mL wash buffer.

    8Wash beads 3× with 1 mL desired storage buffer.

    9.Resuspend in PBS with 0.1% sodium azide (w/v). Store at 4°C (avoid freezing).

C. General Affinity Purification Protocol
Note: Protein purification requires application-specific optimization due to structural diversity.

    1.Transfer optimized bead amount to centrifuge tube. Perform magnetic separation for 1–3 min. Discard supernatant.

        Note: Titrate beads against target protein abundance. Typical binding: 1–20 μg target protein per mg beads. Excess beads increase background; insufficient beads reduce yield.

    2.Wash beads 3× with 5 bead volumes PBS:

            Remove tube from magnet.

            Resuspend in PBS for 30 sec.

            Separate magnetically (1–3 min). Discard supernatant.

    3.Add washed beads to crude sample containing target protein. Incubate 1–2 h at RT or optimized temperature (extend for lower temperatures).

    4.Wash with PBS or 1M NaCl until eluate OD??? < 0.05.

    5.Elute target using:

            Low pH (2–4)

            High pH (10–12)

            High-salt buffer

            Heat denaturation

            Affinity elution

            Boiling in SDS-PAGE loading buffer

                (For research use only!)




More Products
97精品人人A片免费看| 久久久久精99久久久久久| 精品国产一区二区三区卡| 青娱乐91中文| 日韩欧美 一区二区三区| 男生使劲插女生下面视频| 亚洲国产精品无码十八禁| 成人性生生活性生交12| 欧美日韩a在线视频下载| 草处女视频在线免费观看| 国内精品久久久久久久试看| 享受隔壁黑人带来的满足| 国产精品无码专区第一页| 亚洲第一黄色网| 精品欧美一区二区久久久伦| A性视频AAA| ae86老司机在线观看| 精品国产美女久久久久久久| 欧美 日韩 精品 中文| 久久只有这才是精品99| 国产美女福利电影第99| 国产午夜免费区二区三区| 被强开花苞的女明星小说| 日韩一区二区电影免费入口| 好逼天天操天天色综合网| 亚洲欧美一区二区三区图片| 黑人后入插美女下面网址| 欧美性色欧美A在线图片| 美女脱光光操逼淫叫网站| 日韩激情小视频在线观看| 黑人的大屌视频| 中国胖美女另类重口网站| 国产suv精品一区二区2| 山上日美女少妇BB视频| 女神啪啪啪被我顶到高潮| 日本日本熟妇中文字幕在线| se09在线艹大奶粉丝| 欧美亚洲另类图片一二区| 色综合欧美精品视频在线| 欧洲女人性开放免费网站| 亚洲中文字幕一区女教师|