客戶采用我司羧基聚合物磁珠偶聯(lián)抗體捕獲外泌體在《Talanta》發(fā)表論文
客戶采用我司4um羧基磁珠偶聯(lián)anti-CD63抗體，然后用于捕獲外泌體，裂解后反轉(zhuǎn)率等溫?cái)U(kuò)增，用于檢測(cè)。
Qiuyuan Lin, Zhipeng Huang, Xin Ye, Bin Yang, Xueen Fang, Baohong Liu, Hui Chen, Jilie Kong,
Lab in a tube: Isolation, extraction, and isothermal amplification detection of exosomal long noncoding RNA of gastric cancer,
Talanta,
Volume 225,
2021,
122090,
ISSN 0039-9140,
https://doi.org/10.1016/j.talanta.2021.122090.
(http://www.sciencedirect.com.group21-s.aronip.com/science/article/pii/S0039914021000114)
Abstract: Tumor-derived exosomes that inherit molecular information on parental cells hold great promise for cancer diagnostics. Currently, two main technical challenges, time-consuming and labor-intensive isolation of exosome and nucleic acid extraction with limited recovery that have restricted the detection of ultralow abundance exosomal nucleic acids. Here, we proposed a simple, efficient and “l(fā)ab in a tube” system for the detection of exosomal nucleic acids, which fully integrated exosomes enrichment using immunomagnetic beads (IMB) (10 min), fast exosomes lysis based on NP-40 lysate (5 min) and sensitive loop-mediated isothermal amplification (LAMP) in a tube. This method was demonstrated by detecting two exosomal long noncoding RNA biomarkers of gastric cancer (HOTTIP and lncRNA-GC1) with a dynamic detection ranging from 300 ng/μL to 10 ng/μL, and the detection limit of LAMP was 10 ng/μL. Additionally, this platform exhibited good performance in the analysis of exosomal HOTTIP RNA directly in human serum samples, which has the potential for detection of low-abundance exosomal nucleic acid biomarkers from cancers.
Keywords: Exosomal long noncoding RNA; Immunomagnetic beads isolation; Nucleic acid extraction; Loop-mediated isothermal amplification (LAMP)
摘要：腫瘤源性外顯子是遺傳親本細(xì)胞分子信息的重要來(lái)源，對(duì)腫瘤診斷具有重要的應(yīng)用前景。目前，外顯子的耗時(shí)和勞動(dòng)密集分離和回收率有限的核酸提取兩大技術(shù)難題限制了超低豐度外顯子核酸的檢測(cè)。本文提出了一種簡(jiǎn)便、高效、“試管內(nèi)實(shí)驗(yàn)室”的體外核酸檢測(cè)系統(tǒng)，該系統(tǒng)利用免疫磁珠（IMB）（10min）、基于NP-40溶解酶（5min）和敏感環(huán)介導(dǎo)的等溫?cái)U(kuò)增（LAMP）技術(shù)，實(shí)現(xiàn)了完整的胞外體富集。本方法通過(guò)檢測(cè)胃癌（HOTTIP和lncRNA-GC1）兩個(gè)外顯體長(zhǎng)非編碼RNA生物標(biāo)記物，動(dòng)態(tài)檢測(cè)范圍為300ng，證明了該方法的有效性/μL至10 ng/μ五十、 燈的檢測(cè)限為10ng/μL。該平臺(tái)在直接檢測(cè)人血清外周血中的外周熱尖RNA方面表現(xiàn)出良好的性能，有可能檢測(cè)腫瘤低豐度的外顯子核酸生物標(biāo)志物。
關(guān)鍵詞：外顯子長(zhǎng)非編碼RNA；免疫磁珠分離；核酸提取；環(huán)介導(dǎo)等溫?cái)U(kuò)增（LAMP）
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