无码人妻久久一区二区三区免费丨,91精品欧美久久久久久,色肉色伦交av色肉色伦,操逼贱货调教操骚逼视频

聯(lián)系方式 Contact

地址:廈門市集美大道1300號

電話:15805933710

聯(lián)系人:許先生

QQ:1974707632 (節(jié)假日可詢)

微信:15805933710 (節(jié)假日可詢)

郵件:purimag@139.com

在線QQ交談 在線QQ交談

搜索 Search

文章快遞:中日友好醫(yī)院采用我司磁珠實(shí)現(xiàn)深度血清蛋白質(zhì)組學(xué)譜發(fā)現(xiàn)潛在生物標(biāo)志物

2025-5-3 12:38:08點(diǎn)擊:



Tan C, Qin G, Wang Q Q, et al. Comprehensive serum proteomics profiles and potential protein biomarkers for the early detection of advanced adenoma and colorectal cancer[J]. World Journal of Gastrointestinal Oncology, 2024, 16(7): 2971.

Comprehensive serum proteomics profiles and potential protein biomarkers for the early detection of advanced adenoma and colorectal cancer

全面的血清蛋白質(zhì)組學(xué)譜和潛在的蛋白質(zhì)生物標(biāo)志物,用于晚期腺瘤和結(jié)直腸癌的早期檢測

Abstract

BACKGROUND

The majority of colorectal cancer (CRC) cases develop from precursor advanced adenoma (AA). With the development of proteomics technologies, blood protein biomarkers have potential applications in the early screening of AA and CRC in the general population.

大多數(shù)結(jié)直腸癌 (CRC) 病例由前體晚期腺瘤 (AA) 發(fā)展而來。隨著蛋白質(zhì)組學(xué)技術(shù)的發(fā)展,血液蛋白生物標(biāo)志物在普通人群 AA 和 CRC 的早期篩查中具有潛在的應(yīng)用。

AIM

To identify serum protein biomarkers for the early screening of AA and CRC.

確定用于 AA 和 CRC 早期篩查的血清蛋白生物標(biāo)志物。

METHODS

We collected 43 serum samples from 8 normal controls (NCs), 19 AA patients and 16 CRC patients at China-Japan Friendship Hospital. Quantitative proteomic analysis was performed using liquid chromatography–mass spectrometry/mass spectrometry and data independent acquisition, and differentially expressed proteins (DEPs) with P-values < 0.05 and absolute fold changes > 1.5 were screened out, followed by bioinformatics analysis. Prognosis was further analyzed based on public databases, and proteins expression in tissues were validated by immunohistochemistry.

我們從中日友好醫(yī)院的 8 例正常對照 (NCs) 、19 例 AA 患者和 16 例 CRC 患者中收集了 43 份血清樣本。使用液相色譜-質(zhì)譜/質(zhì)譜和數(shù)據(jù)非依賴性采集進(jìn)行定量蛋白質(zhì)組學(xué)分析,篩選出 P 值< 0.05 且絕對倍數(shù)變化> 1.5 的差異表達(dá)蛋白 (DEP),然后進(jìn)行生物信息學(xué)分析?;诠矓?shù)據(jù)庫進(jìn)一步分析預(yù)后,并通過免疫組化驗(yàn)證組織中蛋白質(zhì)的表達(dá)。

RESULTS

A total of 2132 proteins and 17365 peptides were identified in the serum samples. There were 459 upregulated proteins and 118 downregulated proteins in the NC vs AA group, 289 and 180 in the NC vs CRC group, and 52 and 248 in the AA vs CRC group, respectively. Bioinformatic analysis revealed that these DEPs had different functions and participated in extensive signaling pathways. We also identified DIAPH1, VASP, RAB11B, LBP, SAR1A, TUBGCP5, and DOK3 as important proteins for the progression of AA and CRC. Furthermore, VASP (P < 0.01), LBP (P = 0.01), TUBGCP5 (P < 0.01), and DOK3 (P < 0.01) were associated with a poor prognosis. In addition, we propose that LBP and VASP may be more promising protein biomarkers for the early screening of colorectal tumors.

血清樣品中共鑒定出 2132 種蛋白質(zhì)和 17365 種肽。NC vs AA 組有 459 個上調(diào)蛋白和 118 個下調(diào)蛋白,NC vs CRC 組有 289 個和 180 個,AA vs CRC 組有 52 個和 248 個。生物信息學(xué)分析顯示,這些 DEPs 具有不同的功能,并參與廣泛的信號通路。我們還發(fā)現(xiàn) DIAPH1 、 VASP 、 RAB11B 、 LBP 、 SAR1A 、 TUBGCP5 和 DOK3 是 AA 和 CRC 進(jìn)展的重要蛋白。此外,VASP (P < 0.01) 、LBP (P = 0.01) 、TUBGCP5 (P < 0.01) 和 DOK3 (P < 0.01) 與不良預(yù)后相關(guān)。此外,我們提出 LBP 和 VASP 可能是更有前途的蛋白質(zhì)生物標(biāo)志物,用于結(jié)直腸腫瘤的早期篩查。

CONCLUSION

Our study elucidated the serum proteomic profiles of AA and CRC patients, and the identified proteins, such as LBP and VASP, may contribute to the early detection of AA and CRC.

我們的研究闡明了 AA 和 CRC 患者的血清蛋白質(zhì)組學(xué)特征,鑒定出的 LBP 和 VASP 等蛋白可能有助于 AA 和 CRC 的早期檢測。

Keywords: Serum proteomics, Advanced adenoma, Colorectal cancer, Protein biomarker, Early screening

關(guān)鍵字:血清蛋白質(zhì)組學(xué), 晚期腺瘤, 結(jié)直腸癌, 蛋白質(zhì)生物標(biāo)志物, 早期篩查

本論文所使用的高深度蛋白組學(xué)磁珠請參考:http://www.5000js.com/Product/8096211554.html

Sample collection and preparation

Fresh whole blood samples were collected from NCs, AA patients and CRC patients before surgery and centrifuged at 2-8 °C and 3000 r/min for 15 min at room temperature. The supernatant serum was collected into 2 mL freezing tubes and transferred to a -80 °C refrigerator for storage.

The sample preparation steps included low-abundance protein enrichment, protein denaturation, reduction, and alkylation as well as the digestion and peptide cleanup. Briefly, 1 mg of PuriMag magnetic beads (PuriMag Biotech, Xiamen, China) was diluted with 100 μL of wash buffer (10 mmol/L Tris (pH = 7.4), 150 mmol/L KCl, 0.05% CHAPS), and then 100 μL of serum was added. Then, the mixture was incubated at 37 °C for 1 h at 1000 rpm. After incubation, the beads were collected by the magnetic separation device and further washed with 300 μL of wash buffer three times with the magnetic separation device. The precipitate was resuspended in 40 μL of Lyse buffer (0.1 M urea, 5 mmol/L TCEP, 10 mmol/L CAA) and heated at 95 °C for 5 min at 1000 rpm with agitation. After cooling to room temperature, Lys-C and trypsin solution were added, and the sample was incubated at 37 °C for 2 h at 500 rpm with shaking. The digestion process was stopped with 10% TFA. Sample clean-up and desalting were carried out by a C18 peptide cleaning column. Peptides were eluted twice with 30 μL of elution buffer (90% ACN, 0.2% TFA) and then dried in a speed vacuum concentrator.


Proteomic signatures

We identified a total of 2132 proteins and 17365 peptides in the serum samples. 




67194欧美日韩国产| 午夜视频在线看免费蜜桃| 操女生小穴网站| 五十路人妻av高清在线| 日本人妻中文字幕久久一区| 久久伊人色av天堂九九| 一区二区三区国产欧美日本| 黑鸡把插逼视频| 国产三级视频在线2022| 美女夹着大鸡吧啪啪视频| 无码av一区二区免费看| 欧美 国产 在线一区三区| av 中文字幕 一区二区| 中文字幕无线码欧美成人| 日韩黄色人妻呻吟中出视频| 骚逼真爽欲求不满的样子| 印度拍的马操女逼的网站| 人人妻人人澡人人添人人爽| 人妻乱人伦中文字幕在线| 大鸡巴一进一出快乐湿逼| 日韩 无码 中文 另类| 看黄片一级片黄片一级片| 亚洲欧美一区二区爽爽爽| 波多野中文字幕| 暴操小骚逼视频| 男女爽爽爽视频| 色香蕉日韩大片在线观看| 亚洲av综合a色av中文| 茄子视频在线观看你懂的| 天天操天天爱天天操天天爱 | 被吃奶抽插啊啊啊啊啊啊| 日韩无码小视频| 国产精品久久九九精品。| 黄色视频网站在线观看污| yw193尤物在线网站| 欧美亚洲另类图片与小说| 午夜乱人伦精品视频在线| 久久久国产精品夜夜夜夜| 蜜臀aⅴ精品一区二区三区| 大逼前入式动态抽插视频| 无码在线日嫩V|