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客戶采用我公司硅羥基磁珠提取COVID-19新冠病毒RNA，然后不洗脫直接帶磁珠進(jìn)行等溫?cái)U(kuò)增RT-LAMP，用于新冠病毒的檢測(cè)。

Yugan He, Tie Xie, Yigang Tong,
Rapid and highly sensitive one-tube colorimetric RT-LAMP assay for visual detection of SARS-CoV-2 RNA,
Biosensors and Bioelectronics,
Volume 187,
2021,
113330,
ISSN 0956-5663,
https://doi.org/10.1016/j.bios.2021.113330.
(http://www.sciencedirect.com.group21-s.aronip.com/science/article/pii/S0956566321003675)

Abstract: Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is a highly contagious disease. To tame the continuously raging outbreak of COVID-19, developing a cheap, rapid and sensitive testing assay is absolutely imperative. Herein, we developed a one-tube colorimetric RT-LAMP assay for the visual detection of SARS-CoV-2 RNA. The assay integrates Si–OH magnetic beads (MBs)-based fast RNA extraction and rapid isothermal amplification in a single tube, thus bypassing the RNA elution step and directly amplifying on-beads RNA molecules with the visualized results. This one-tube assay has a limit of detection (LOD) as low as 200 copies/mL for sample input volumes of up to 600 μL, and can be performed in less than 1 h from sample collection to result readout. This assay demonstrated a 100% concordance with the gold standard test RT-qPCR test by using 29 clinical specimens and showed high specificity. This one-tube colorimetric RT-LAMP assay can serve as an alternative platform for a rapid and sensitive diagnostic test for COVID-19 and is particularly suitable for use at community clinics or township hospitals.
Keywords: One-tube; SARS-CoV-2; LAMP; Point-of-care test; Visual readout


摘要：冠狀病毒病（COVID-19）是由SARS-CoV-2（嚴(yán)重急性呼吸綜合征冠狀病毒2型）引起的一種高度傳染性疾病。為了遏制持續(xù)肆虐的COVID-19病毒爆發(fā)，開發(fā)一種廉價(jià)、快速、靈敏的檢測(cè)方法勢(shì)在必行。在此，我們開發(fā)了一種單管比色RT-LAMP檢測(cè)SARS-CoV-2rna的方法。該方法將基于硅-羥基磁珠（MBs）的快速RNA提取和快速等溫?cái)U(kuò)增集成在一個(gè)試管中，從而繞過RNA洗脫步驟，直接在珠狀RNA分子上擴(kuò)增，結(jié)果可視化。這種單管分析的檢測(cè)限（LOD）低至200拷貝/mL，樣品輸入量高達(dá)600μ五十、 從樣品采集到結(jié)果讀出不到1小時(shí)。用29例臨床標(biāo)本進(jìn)行RT-qPCR檢測(cè)，結(jié)果與金標(biāo)準(zhǔn)試驗(yàn)符合率為100%，具有較高的特異性。這種單管比色RT-LAMP檢測(cè)方法可以作為COVID-19快速敏感診斷試驗(yàn)的替代平臺(tái)，特別適合在社區(qū)診所或鄉(xiāng)鎮(zhèn)醫(yī)院使用。

關(guān)鍵詞：?jiǎn)喂?；SARS-CoV-2；燈；護(hù)理點(diǎn)測(cè)試；視覺讀數(shù)


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