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針對(duì)全球首個(gè)生物納米磁珠國(guó)際標(biāo)準(zhǔn)ISO - TS 19807-2的評(píng)述

2022-11-5 12:16:21點(diǎn)擊:



納米技術(shù)-磁性納米材料-第2部分:核酸提取用納米結(jié)構(gòu)磁性微球特性和測(cè)量方法規(guī)范

2021年10月,國(guó)際標(biāo)準(zhǔn)化組織官方網(wǎng)站發(fā)布了第一個(gè)生物納米磁珠國(guó)際標(biāo)準(zhǔn):ISO/TS 19807-2:2021納米技術(shù)-磁性納米材料-第2部分:核酸提取用納米結(jié)構(gòu)磁珠特性和測(cè)量方法規(guī)范,該標(biāo)準(zhǔn)對(duì)規(guī)范和推動(dòng)磁珠在生物醫(yī)學(xué)領(lǐng)域的應(yīng)用具有重要的意義。

ISO - TS 19807-2

Nanotechnologies — Magnetic nanomaterials — Part 2: Specification of characteristics and measurement methods for nanostructured magnetic beads for nucleic acid extraction

該專利的核心部分如下:

3   Terms and definitions
For the purposes of this document, the terms and definitions given in ISO/TS 80004-1, ISO/TS 80004-6 and the following apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp
— IEC Electropedia: available at http://www.electropedia.org/
3.1
bead mass concentration 粒子質(zhì)量濃度
ratio of the mass of magnetic beads (3.6) to the total volume of a magnetic beads sample in suspension or powder form
3.2
bead size 粒子尺寸
effective outer diameter of a magnetic bead (3.6) determined by using the specified measurement method
3.3
bead size distribution 粒子尺寸分布
distribution of beads as a function of bead size (3.2)
Note 1 to entry: Bead size distribution may be expressed as cumulative distribution or a distribution density (distribution of the fraction of beads in a size class, divided by the width of that class).
3.4
dispersing medium 分散介質(zhì)
liquid in which magnetic beads (3.6) are suspended
3.5
initial magnetic mass susceptibility 磁性粒子矯頑力
differential ratio of the change in mass magnetization of a material to the amplitude of a magnetic field change at a sufficiently small absolute magnetic field
Note 1 to entry: A magnetic beads (3.6) sample is assumed to be magnetically isotropic and its initial magnetic mass susceptibility is indicated as a scalar.
3.6
magnetic bead 磁珠
small round piece containing a large number of magnetic nanoparticles which can be superparamagnetic and are immobilized within a non-magnetic matrix
Note 1 to entry: The size range of magnetic beads for DNA extraction spans from a few tens of nanometres to several micrometres.
3.7
mass-specific surface area 單位質(zhì)量比表面積
absolute surface area of the sample divided by sample mass
[SOURCE:ISO/TS 80004-6:2021, 4.6.1, modified — Note 1 to entry has been removed.]
3.8
nucleic acid 核酸
macromolecule that is the medium for genetic information or acts as an agent in expressing the information
Note 1 to entry: There are two types of nucleic acid, DNA and RNA.
[SOURCE:ISO 17822:2020, 3.32]
3.9
nucleic acid binding capacity 核酸結(jié)合容量
mass of nucleic acid (3.8) bound to the surfaces of magnetic beads (3.6) per unit mass of the magnetic beads under specified conditions
3.10
operational time 操作時(shí)間
maximum time after the start of the extraction process where the suspension of magnetic beads (3.6) is ready for use to extract nucleic acid (3.8)
Note 1 to entry: the operational time is usually recommended by the manufacturer.
3.11
remanent mass magnetization 剩磁
value of the mass magnetization remaining in a magnetized body when, in the absence of a self-demagnetizing field, the applied magnetic field strength is brought to zero
[SOURCE:IEC 60050:1990, 221-02-40, modified — "magnetization" has been changed to "mass magnetization".]
3.12
saturation mass magnetization 飽和磁強(qiáng)度
limiting value of the mass magnetization of a liquid or dried sample with increasing applied magnetic field strength
Note 1 to entry: The saturation mass magnetization of magnetic beads (3.6) is indicated for the dried matter of a bead suspension sample or for the dried sample in the case of beads in powder form.
3.13
shelf life 貨架期
recommended time period by manufacturer during which a product (suspension or powder) can be stored, throughout which the defined quality of specified characteristics of the product remains acceptable under expected (or specified) conditions of distribution, storage, display and usage
Note 1 to entry: Defined characteristics should be measured after fixed time intervals.
[SOURCE:ISO/TS 19807-1:2019, 3.37, modified — the manufacturer has been specified and the powder product has been added.]
3.14
surface functional group density 表面功能基團(tuán)密度
mass of surface functional groups per unit mass of magnetic beads (3.6)
3.15
surface functional group type 表面功能基團(tuán)類型
chemical type of substituents or moieties on the surface of magnetic beads (3.6) that are responsible for a specific chemical reaction

簡(jiǎn)單來(lái)說,就是這個(gè)專利規(guī)范了磁性納米粒子的一些標(biāo)準(zhǔn)特征和這些特征的檢測(cè)方法。

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