客戶利用我司G-COOH磁珠偶聯(lián)噬菌體開發(fā)檢測副溶血性弧菌技術(shù)在Food Chemistry 發(fā)表論文
Qiaoling Du, et al. A novel phagomagnetic separation-ATP bioluminescence (PhMS-BL) for rapid and sensitive detection of viable Vibrio parahaemolyticus in aquatic product, Food Chemistry, Volume 439, 1 May 2024, 138113. https://doi.org/10.1016/j.foodchem.2023.138113 (G-COOH磁珠偶聯(lián)噬菌體檢測副溶血性弧菌)
A novel phagomagnetic separation-ATP bioluminescence (PhMS-BL) for rapid and sensitive detection of viable Vibrio parahaemolyticus in aquatic product
Abstract
Detection of viable Vibrio parahaemolyticus (V. parahaemolyticus) is a major challenge due to its significant risk to food safety and human health. Herein, we developed a phagomagnetic separation-ATP bioluminescence (PhMS-BL) assay based on phage VPHZ6 for rapid and sensitive detection of viable V. parahaemolyticus. Phage as a recognition element was coupled to magnetic beads to capture and enrich V. parahaemolyticus, shortening detection time and improving method sensitivity. The intracellular ATP released by chemical lysis using CTAB was quantified using firefly fluorescein-adenosine triphosphate bioluminescence system to detect viable bacteria. So, PhMS-BL method was able to detect V. parahaemolyticus in a linear range of 2.3 × 102 to 1.3 × 107 CFU mL?1, with a detection limit of 78 CFU mL?1 within 15 min. It is successfully applied to detect V. parahaemolyticus in spiked lake water, lobster tail meat, and clam meat. The developed detection strategy can rapidly and sensitively detect viable V. parahaemolyticus in food matrixes.
新型噬磁分離-ATP生物發(fā)光（PhMS-BL）用于快速、靈敏地檢測水產(chǎn)品中活的副溶血性弧菌
Food Chemistry ( IF 8.8 ) Pub Date : 2023-11-30 , DOI: 10.1016/j.foodchem.2023.138113
Qiaoling Du, Yifeng Ding, Yuanshang Wang, Jia Wang, Yanchun Shao, Xiaohong Wang
活副溶血弧菌（V. parahaemolyticus ）的檢測是一項(xiàng)重大挑戰(zhàn)，因?yàn)樗鼘κ称钒踩腿祟惤】禈?gòu)成重大風(fēng)險。在此，我們開發(fā)了一種基于噬菌體 VPHZ6 的噬磁分離-ATP 生物發(fā)光 (PhMS-BL) 測定法，用于快速、靈敏地檢測活副溶血性弧菌。噬菌體作為識別元件與磁珠偶聯(lián)，捕獲并富集副溶血弧菌，縮短檢測時間，提高方法靈敏度。使用螢火蟲熒光素-三磷酸腺苷生物發(fā)光系統(tǒng)對 CTAB 化學(xué)裂解釋放的細(xì)胞內(nèi) ATP 進(jìn)行定量，以檢測活細(xì)菌。因此，PhMS-BL方法能夠在2.3 × 10 2至1.3 × 10 7 CFU mL -1的線性范圍內(nèi)檢測副溶血性弧菌，在15分鐘內(nèi)檢測限為78 CFU mL -1。它已成功應(yīng)用于檢測加標(biāo)湖水、龍蝦尾肉和蛤肉中的副溶血弧菌。所開發(fā)的檢測策略可以快速、靈敏地檢測食品基質(zhì)中的活副溶血弧菌。
羧基磁珠請參考：羧基磁珠|高密度羧基磁珠|聚合物羧基磁珠PuriMag G-COOH|carboxyl magnetic bead-生物磁珠專家 (purimagbead.com)
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